CCR5Δ32 (rs333) polymorphism is associated with decreased risk of chronic and aggressive periodontitis: A case-control analysis based in disease resistance and susceptibility phenotypes. (March 2018)

• Record Type: Journal Article
• Authors: Cavalla, Franco; Biguetti, Claudia C.; Dionisio, Thiago J.; Azevedo, Michelle C.S.; Martins Jr, Walter; Santos, Carlos F.; Trombone, Ana Paula F.; Silva, Renato M.; Letra, Ariadne; Garlet, Gustavo P.
• Is Part Of: Cytokine. Volume 103(2018)
• Page Start: 142
• Page End: 149
• Publication Date: 2018-03
• Subjects: Periodontitis -- CCR5Δ32 -- Inflammation -- Pathogens; Cytokines -- Periodicals; 571.844
• DOI: 10.1016/j.cyto.2017.09.022
• Languages: English
• ISSNs: 1043-4666

Abstract

Abstract: Chronic and aggressive periodontitis are infectious diseases characterized by the irreversible destruction of periodontal tissues, which is mediated by the host inflammatory immune response triggered by periodontal infection. The chemokine receptor CCR5 play an important role in disease pathogenesis, contributing to pro-inflammatory response and osteoclastogenesis. CCR5Δ32 (rs333) is a loss-of-function mutation in the CCR5 gene, which can potentially modulate the host response and, consequently periodontitis outcome. Thus, we investigated the effect of the CCR5Δ32 mutation over the risk to suffer periodontitis in a cohort of Brazilian patients (total N = 699), representative of disease susceptibility (chronic periodontitis, N = 197; and aggressive periodontitis, N = 91) or resistance (chronic gingivitis, N = 193) phenotypes, and healthy subjects (N = 218). Additionally, we assayed the influence of CCR5Δ32 in the expression of the biomarkers TNFα, IL-1β, IL-10, IL-6, IFN-γ and T-bet, and key periodontal pathogens P. gingivalis, T. forsythia, and T. denticola. In the association analysis of resistant versus susceptible subjects, CCR5Δ32 mutant allele-carriers proved significantly protected against chronic (OR 0.49; 95% CI 0.29–0.83; p-value 0.01) and aggressive (OR 0.46; 95% CI 0.22–0.94; p-value 0.03) periodontitis. Further, heterozygous subjects exhibited significantly decreased expression of TNFα in periodontal tissues, pointing to a functional effect of the mutation in periodontal tissues during the progression of the disease. Conversely, no significant changes were observed in the presence or quantity of the periodontal pathogens P. gingivalis, T. forsythia, and T. denticola in the subgingival biofilm that could be attributable to the mutant genotype.