Ameliorative effects of Juniperus rigida fruit on oxazolone- and 2, 4-dinitrochlorobenzene-induced atopic dermatitis in mice. (25th March 2018)

• Record Type: Journal Article
• Authors: Lee, Sullim; Park, No-June; Bong, Sim-Kyu; Jegal, Jonghwan; Park, Sang-a; Kim, Su-Nam; Yang, Min Hye
• Is Part Of: Journal of ethnopharmacology. Volume 214(2018)
• Page Start: 160
• Page End: 167
• Publication Date: 2018-03-25
• Subjects: JFE Juniperus rigida fruit EtOH extract -- AD atopic dermatitis -- DNCB 2, 4-dinitrochlorobenzene -- IL-4 interleukin-4 -- IgE immunoglobulin E -- Th2 T-helper cell type-2 -- TEWL transepidermal water loss -- PMA phorbol 12-myristate 13-acetate; Juniperus rigida fruit -- Atopic dermatitis -- Skin barrier function -- Interleukin 4 -- Cupressuflavone -- Isoscutellarein-7-O-β-xylopyranoside; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2017.12.022
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: The fruits of Juniperus rigida have been used in Korean traditional medicine for the treatment of inflammatory diseases in humans such as rheumatoid arthritis. Aim of the study: This study aimed to investigate the anti-atopic properties of J. rigida fruit in in vivo murine atopic dermatitis (AD) models. Methods and results: BALB/c mouse ears ad SKH-1 hairless mice stimulated with oxazolone (4 weeks) and DNCB (3 weeks), respectively, were treated with the 1% Juniperus rigida fruit EtOH extract (JFE). The JFE improved AD symptoms in both oxazolone- and DNCB-induced AD mice by accelerating skin barrier recovery function and suppressing the overproduction of serum immunoglobulin E (IgE) and interleukin 4 (IL-4). The JFE was found to contain isoscutellarein-7- O -β-xylopyranoside, cupressuflavone, podocarpusflavone A, and hinokiflavone as major components based on phytochemical analysis. Eight flavonoids were isolated from JFE, and of those, cupressuflavone and isoscutellarein-7- O -β-xylopyranoside strongly down-regulated IL-4 expression and β-hexosaminidase release in RBL-2H3 cells. Conclusion: Therapeutic attempts with J. rigida fruit and its active components might be useful in treating AD and related skin inflammatory diseases. Graphical abstract: