Cultivation and quantitative single‐cell analysis of Saccharomyces cerevisiae on a multifunctional microfluidic device. Issue 3 (4th October 2017)

• Record Type: Journal Article
• Authors: Stratz, Simone; Verboket, Pascal Emilio; Hasler, Karina; Dittrich, Petra Stephanie
• Editors: Foret, Frantisek; Kutter, Jörg P.
• Is Part Of: Electrophoresis. Volume 39:Issue 3(2018)
• Page Start: 540
• Page End: 547
• Publication Date: 2017-10-04
• Subjects: Clonal cell culture -- Microfluidics -- Saccharomyces cerevisiae -- Single‐cell analysis; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201700253
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: Here, we present a multifunctional microfluidic device whose integrative design enables to combine cell culture studies and quantitative single cell biomolecule analysis. The platform consists of 32 analysis units providing two key features; first, a micrometer‐sized trap for hydrodynamic capture of a single Saccharomyces cerevisiae ( S. cerevisiae ) yeast cell; second, a convenient double‐valve configuration surrounding the trap. Actuating of the outer valve with integrated opening results in a partial isolation in a volume of 11.8 nL, i.e. the cell surrounding fluid can be exchanged diffusion‐based without causing shear stress or cell loss. Actuation of the inner ring‐shaped valve isolates the trapped cell completely in a small analysis volume of 230 pL. The device was used to determine the growth rate of yeast cells ( S. cerevisiae ) under under optimum and oxidative stress conditions. In addition, we successfully quantified the cofactor beta‐nicotinamide adenine dinucleotide phosphate (NAD(P)H) in single and few cells exposed to the different microenvironments. In conclusion, the microdevice enables to analyze the influence of an external stress factor on the cellular fitness in a fast and more comprehensive way as cell growth and intracellular biomolecule levels can be investigated.