The effect of dental bleaching on pulpal tissue response in a diabetic animal model: a study of immunoregulatory cytokines. (25th September 2017)

• Record Type: Journal Article
• Authors: Ferreira, L. L.; Gomes‐Filho, J. E.; Benetti, F.; Carminatti, M.; Ervolino, E.; Briso, A. L. F.; Cintra, L. T. A.
• Is Part Of: International endontic journal. Volume 51:Number 3(2018)
• Page Start: 347
• Page End: 356
• Publication Date: 2017-09-25
• Subjects: dental bleaching -- diabetes mellitus -- inflammatory response -- proinflammatory cytokines; Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12852
• Languages: English
• ISSNs: 0143-2885

Abstract

Abstract: Aim: To evaluate the influence of tooth bleaching on immunoregulatory cytokines production (IL‐6, Tumour necrosis factor (TNF)‐α and IL‐17) in the pulp tissue of normoglycaemic and diabetic rats. Methodology: Twenty‐eight rats were divided into normoglycaemic and diabetic rats ( n = 14). Diabetes mellitus (DM) was induced with a single dose of alloxan diluted in citrate buffer via intramuscular injection. After DM confirmation, all rats were sedated and tooth bleaching was performed using 35% hydrogen peroxide on the right maxillary molars for 30 min. Left molars were used as controls. Bleaching resulted in four hemimaxillae groups: normoglycaemic (N), N‐bleached (NBle), diabetic (D) and D‐bleached (DBle). After 2 and 30 days, rats were euthanized and hemimaxillae processed for analysis by haematoxylin and eosin and immunohistochemistry. Results within and between animals were submitted to Wilcoxon signed‐rank and Mann–Whitney tests ( P < 0.05). Results: At 2 days, the NBle group had mild, and the DBle had severe inflammatory infiltration in the pulpal tissue ( P < 0.05). TNF‐α and IL‐6 cytokines were associated with increased immunolabelling in the bleached groups compared to nonbleached ( P < 0.05). However, IL‐17 had increased immunolabelling in the NBle compared to the N and DBle group ( P < 0.05). At 30 days, reactionary dentine was observed in the coronal pulp of all bleached teeth and no inflammation was present ( P > 0.05). TNF‐α cytokines had increased immunolabelling in the DBle group compared to the D group ( P < 0.05). However, for IL‐6 and IL‐17, no difference was observed in this period ( P > 0.05). Conclusions: Tooth bleaching increased IL‐6 and TNF‐α in the pulp tissue regardless of diabetes mellitus; however, diabetic rats had higher TNF‐α levels for longer periods. Tooth bleaching influenced the increase in IL‐17 in the early periods in normoglycaemic rats.