Application of high‐speed counter‐current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum. Issue 3 (21st December 2017)

• Record Type: Journal Article
• Authors: Chen, Bao; Liu, Zhengbo; Zhang, Yuwei; Li, Wei; Sun, Yanjun; Wang, Yufang; Wang, Yanhua; Sun, Yinshi
• Is Part Of: Journal of separation science. Volume 41:Issue 3(2018)
• Page Start: 789
• Page End: 796
• Publication Date: 2017-12-21
• Subjects: high‐speed counter‐current chromatography -- lobetyolin -- platetyolin -- polyacetylenes -- traditional Chinese medicine; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201700767
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: Three polyacetylenes were isolated and purified from Platycodon grandiflorum A. DC for the first time by high‐speed counter‐current chromatography using a two‐phase solvent system composed of hexane/ethyl acetate/methanol/water (1:31:1:31, v/v/v/v) and high‐performance liquid chromatography with an Agilent ZORBAX® SB‐C18 column (4.6 mm × 150 mm, 5 μm). After separation by high‐speed counter‐current chromatography and high‐performance liquid chromatography, we obtained 3.5 mg of platetyolin A, 4.1 mg of platetyolin B, and 18.1 mg of lobetyolin with purities of 97.2, 96.7, and 96.9%, respectively. The purity of each compound was assessed by high‐performance liquid chromatography and the chemical structures were evaluated by high‐resolution electrospray ionization time‐of‐flight mass spectrometry and one‐ and two‐dimensional NMR spectroscopy. Among the isolated compounds, platetyolin A and platetyolin B are newly reported compounds.