A Luminescent Perylenediimide as a Binding Ligand for Pyrimidine/Pyrimidine Mismatches Within a DNA Duplex. Issue 21 (25th July 2017) - Takada, Tadao
Abstract: Synthetic small molecules with a binding affinity to mismatched base pairs have been widely studied because of their potential applications in chemotherapeutic drugs and diagnostic tools for the detection of single nucleotide variations. In this paper, a luminescent ligand, a perylenediimide (PO ) derivative, which is capable of binding to mismatched base pairs within a DNA duplex and generating a strong fluorescence upon the binding event, was demonstrated. Fluorescence enhancement ofPO upon the binding to pyrimidine/pyrimidine mismatches were prominent and reached ca. 100‐fold for a C/C mismatched base pair, which was visually observed. This result represents the best performance as a fluorescence turn‐on response among the existing mismatch binding ligands. Abstract : A luminescent ligand, a perylenediimide (PO) derivative, which is capable of binding to mismatched base pairs within a DNA duplex and generating a strong fluorescence upon the binding event, was demonstrated. Fluorescence enhancement of PO upon the binding to pyrimidine/pyrimidine mismatches were prominent and reached ca. 100‐fold for a C/C mismatched base pair, which was visually observed.