Anti-inflammatory effect of litsenolide B2 isolated from Litsea japonica fruit via suppressing NF-κB and MAPK pathways in LPS-induced RAW264.7 cells. (March 2015)

• Record Type: Journal Article
• Authors: Ham, Young-Min; Ko, Yeong-Jong; Song, Sang-Mock; Kim, Jihyun; Kim, Kil-Nam; Yun, Ji-Hyun; Cho, Ju-Hyun; Ahn, Ginnae; Yoon, Weon-Jong
• Is Part Of: Journal of functional foods. Volume 13(2015)
• Page Start: 80
• Page End: 88
• Publication Date: 2015-03
• Subjects: Litsea japonica fruit -- Litsenolide -- Anti-inflammatory -- NF-κB -- MAPKs; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2014.12.031
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: We study the anti-inflammatory effect of LNB2 isolated from Litsea japonica fruit. LNB2 inhibited production of inflammatory mediators in LPS-induced RAW 264.7 cells. LNB2 inhibited the LPS-induced phosphorylation of NF-κB and MAPKs. Abstract: The anti-inflammatory effects of compounds isolated from the Litsea japonica fruit were investigated in LPS-stimulated murine macrophage (RAW 264.7) cells. The results indicated that litsenolide (LN)B2 significantly inhibited the LPS-induced release of pro-inflammatory mediators, such as NO and PGE2 . LNC2, and, on the other hand, exhibited cytotoxicity at the same concentrations at which it exhibited an inhibitory property. Therefore, only LNB2 was used for further experimentation. LNB2 reduced the LPS-induced production of pro-inflammatory cytokines. We further investigated the mechanism by which LNB2 inhibits pro-inflammatory mediators and cytokines by examining the level of NF-κB and MAPKs phosphorylation, which all serve as key components in inflammation-induced signaling pathways in RAW 264.7 cells. LNB2 inhibited the LPS-induced phosphorylation of NF-κB and MAPKs. These results suggest that LNB2 has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators and cytokines via inhibition of NF-κB and MAPK signaling in LPS-stimulated RAW 264.7 cells.