Egg-derived ACE-inhibitory peptides IQW and LKP reduce blood pressure in spontaneously hypertensive rats. (March 2015)

• Record Type: Journal Article
• Authors: Majumder, Kaustav; Chakrabarti, Subhadeep; Morton, Jude S.; Panahi, Sareh; Kaufman, Susan; Davidge, Sandra T.; Wu, Jianping
• Is Part Of: Journal of functional foods. Volume 13(2015)
• Page Start: 50
• Page End: 60
• Publication Date: 2015-03
• Subjects: ACE angiotensin converting enzyme -- RAS renin angiotensin system -- Ang II angiotensin II -- CVDs cardiovascular diseases -- SHR spontaneously hypertensive rats -- BP blood pressure -- MAP mean arterial pressure -- SBP systolic blood pressure -- DBP diastolic blood pressure -- HR heart rate -- PE phenylephrine -- MCh methacholine -- L-Name N-nitro-l-arginine methyl ester -- ICAM-1 intercellular cell adhesion molecule-1 -- VCAM-1 vascular adhesion molecule-1 -- NOS nitric oxide synthase -- eNOS endothelial nitric oxide synthase -- NO nitric oxide -- ELISA enzyme-linked immunosorbent assay; Spontaneously hypertensive rats -- Ovotransferrin -- Antihypertensive peptides -- Angiotensin converting enzyme -- Blood pressure; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2014.12.028
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Egg derived tri-peptides IQW and LKP exerted antihypertensive effects on spontaneously hypertensive rats (SHR). Both peptides reduced plasma Ang II (Angiontein II) level and preserved nitric oxide dependent vasorelaxation. IQW, but not LKP, reduced the markers of inflammation and oxidative/nitrosative stress. Abstract: Two angiotensin converting enzyme (ACE) inhibitory tripeptides, IQW and LKP, were previously characterized from egg white protein ovotransferrin. ACE is a key enzyme of the renin–angiotensin system (RAS) which generates angiotensin II (Ang II) from its precursor and increases blood pressure (BP) in the body. This study tested the blood pressure lowering potential of orally administered IQW and LKP in spontaneously hypertensive rats. IQW and LKP treatment decreased mean blood pressure (MAP) by ~19 and ~30 mmHg, respectively, compared to untreated SHRs. The change in BP was accompanied by the preservation of nitric oxide dependent vasorelaxation and lowering of plasma Ang II levels. Furthermore IQW, but not LKP, also reduced intercellular adhesion molecule-1 (ICAM-1) expression and nitrotyrosine levels in arteries, suggesting additional protective effects against inflammation and oxidative/nitrosative stress. These results demonstrate anti-hypertensive effects of IQW and LKP in vivo and a reduction of circulating Ang II levels, with additional anti-inflammatory and antioxidant effects mediated by IQW.