Anti-inflammatory effects of triterpenes and steroid compounds isolated from the stem bark of Hiptage benghalensis. (January 2015)

• Record Type: Journal Article
• Authors: Hsu, Chin-Lin; Fang, Song-Chwan; Huang, Hsiao-Wen; Yen, Gow-Chin
• Is Part Of: Journal of functional foods. Volume 12(2015)
• Page Start: 420
• Page End: 427
• Publication Date: 2015-01
• Subjects: COX-2 cyclooxygenase-2 -- DMSO dimethylsulfoxide -- iNOS inducible nitric oxide synthase -- IL-1β interleukin-1β -- LPS lipopolysaccharide -- MTT 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide -- NO nitric oxide -- NOS nitric oxide synthase -- PBS phosphate buffered saline -- PGE2 prostaglandins E2 -- PVDF polyvinyldifluoride -- SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis -- TNF-α tumor necrosis factor-α; Hiptage benghalensis -- Anti-inflammation -- RAW 264.7 cell -- Triterpenes -- NF-κB; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2014.12.009
• Languages: English
• ISSNs: 1756-4646

Abstract

Research Highlights: Isolation of seven triterpenes and a steroid derivative from the stem bark of Hiptage benghalensis. Compounds 1, 2, and 5–8 suppress inflammation by modulating iNOS and COX-2 expressions. Compounds 1, 2, 7, and 8 exhibit anti-inflammatory activity through the suppression of NF-κB. Abstract: In this study, we investigated the anti-inflammatory effects of triterpenes and steroid derivative isolated from the stem bark of Hiptage benghalensis in LPS-stimulated RAW 264.7 macrophages. In the extraction and isolation section, the chloroform-soluble fraction of the stem bark of H. benghalensis led to the isolation of seven triterpenes and a steroid derivative. These compounds were characterized as alnus-5(10)-en-3β-yl acetate (1 ), oleanan-3-one (2 ), 3β-acetoxy-9β-bauer-7-en-6-one (3 ), lupeol (4 ), (24R)-24-propylcholesterol (5 ), alnus-5(10)-en-3β-ol (6 ), 3β-acetoxy-20-hydroxylupane (7 ), and betulonic acid (8 ) by spectroscopic methods. In vitro anti-inflammatory activity, compounds1, 2, 5, 6, 7, and8 significantly inhibited the productions of NO and PGE2, and protein expressions of iNOS and COX-2 in LPS-stimulated RAW 264.7 macrophages. Compounds1, 2, 7, and8 also significantly increased the protein expression of IκBα, and decreased the protein expression of p-p65 and transcriptional activity of NF-κB in LPS-stimulated RAW 264.7 macrophages. Taken together, the present study clearly demonstrates that compounds isolated from the stem bark of H. benghalensis exhibit anti-inflammatory activity through the suppression of NF-κB in LPS-stimulated RAW 264.7 macrophages.