Determination of total polyphenols in tea by a flow injection-fiber optic spectrophotometric system. Issue 2 (4th March 2018)

• Record Type: Journal Article
• Authors: Qadir, Muyessar; Muhammad, Turghun; Bakri, Mahinur; Gao, Fei
• Is Part Of: Instrumentation science & technology. Volume 46:Issue 2(2018)
• Page Start: 185
• Page End: 193
• Publication Date: 2018-03-04
• Subjects: Fiber optic spectrophotometry -- flow injection analysis -- tea -- total polyphenols; Scientific apparatus and instruments -- Periodicals; 681.2
• DOI: 10.1080/10739149.2017.1346515
• Languages: English
• ISSNs: 1073-9149

Abstract

ABSTRACT: A flow injection-fiber optic spectrophotometric system was established for the online determination of total polyphenol content in tea samples. The method was based on the chromogenic reaction between the phenolic compounds (gallic acid) and ferrous tartrate which was used as the colorimetric reagent and the absorption was determined at the maximum absorption wavelength of 540 nm at 20°C and pH 7.5. The calibration curve from standard solutions of gallic acid was linear in the range from 0.010 to 0.100 mg/mL with a correlation coefficient of 0.9970. The relative standard deviation of polyphenol measurements for eight tea samples was between 0.55 and 3.18%. It can be concluded that the proposed method has allowed simple, fast, and accurate determination of total polyphenol content in tea products.