Unveiling the Dual Role of the Dopaminergic System on Locomotion and the Innate Value for an Aversive Olfactory Stimulus in Drosophila. (10th February 2018)

• Record Type: Journal Article
• Authors: Fuenzalida-Uribe, Nicolás; Campusano, Jorge M.
• Is Part Of: Neuroscience. Volume 371(2018)
• Page Start: 433
• Page End: 444
• Publication Date: 2018-02-10
• Subjects: 3-Oct 3-octanol -- Bz Benzaldehyde -- MB Mushroom Body -- MBONs Mushroom Body output neurons -- P.I. Performance Index -- PAM Protocerebral anterior medial -- PPL1 Protocerebral posterior lateral 1 -- PPL1-MP1 Medial Posterior 1 neurons of the Protocerebral posterior lateral 1 -- Tetx tetanus toxin -- TrpA1 Transient receptor potential cation channel A1; Drosophila -- dopaminergic neurons -- odor valence -- motor programs; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2017.12.032
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Dopaminergic systems innervating the MB lobes modulate innate aversion to odorants. Aversion consists of two components: the assessment of the innate value for an odorant and locomotor response. Neurons in PAM and PPL1 dopamine clusters differentially affect these components. PPL1-MP1 neurons only contribute to innate value for odorant. PAM subpopulations differentially contribute to the two components. Abstract: The communication between sensory systems and the specific brain centers that process this information is crucial to develop adequate behavioral responses. Modulatory systems, including dopaminergic circuits, regulate this communication to finely tune the behavioral response associated to any given stimulus. For instance, the Mushroom Body (MB), an insect brain integration center that receives and processes several sensory stimuli and organizes the execution of motor programs, communicates with MB output neurons (MBONs) to develop behavioral responses associated to olfactory stimuli. This communication is modulated by dopaminergic neural systems. Here we show that silencing dopaminergic neurons increases the aversive response observed in adult flies exposed to Benzaldehyde (Bz) or octanol. We studied the contribution of two dopaminergic clusters that innervate different zones of MB, Protocerebral anterior medial (PAM) and Protocerebral posterior lateral 1 (PPL1), on the innate value to the aversive stimulus and the associated locomotor behavior. In order to do this, we manipulated the synaptic transmission of these neural clusters through the expression of Tetanus toxin, Kir2.1 and Transient receptor potential cation channel A1 (TrpA1) channels. Our results show that neurons in PPL1 and PAM differentially modulate the innate value to Bz in adult flies. On the other hand, blocking neurotransmission or genetic silencing of PAM neurons results in decreased locomotor behavior in flies, an effect not observed when silencing PPL1. Our results suggest that as in mammals, specific dopaminergic pathways differentially modulate locomotor behavior and the innate value for an odorant, a limbic-like response in Drosophila .