Identification of genes involved in the four stages of colorectal cancer: Gene expression profiling. (February 2018)

• Record Type: Journal Article
• Authors: Shi, Guiling; Wang, Yijia; Zhang, Chunze; Zhao, Zhenying; Sun, Xiuying; Zhang, Shiwu; Fan, Jinling; Zhou, Cunxia; Zhang, Jihong; Zhang, Huijuan; Liu, Jun
• Is Part Of: Molecular and cellular probes. Volume 37(2018)
• Page Start: 39
• Page End: 47
• Publication Date: 2018-02
• Subjects: Cancer stage -- Colorectal cancer -- Functional interaction -- Protein-protein interaction -- Pathway enrichment analysis; Molecular probes -- Diagnostic use -- Periodicals; Pathology, Cellular -- Technique -- Periodicals; Cell Biology -- Periodicals; Molecular Biology -- Periodicals; Sondes moléculaires -- Utilisation diagnostique -- Périodiques; Cytopathologie -- Technique -- Périodiques; 572
• DOI: 10.1016/j.mcp.2017.11.004
• Languages: English
• ISSNs: 0890-8508

Abstract

Abstract: Background: Colorectal cancer (CRC) is a common cancer with high morbidity and mortality. However, its molecular mechanism is not clear, nor the genes related to CRC stages. Methods: Gene expression data in CRC and healthy colorectal tissues were obtained from gene expression omnibus. Limma package was used to identify the differentially expressed genes (DEGs) between control and CRC (stage I, II, III, and IV), obtaining 4 DEG sets. VennPlex was utilized to find all DEGs and intersection DEGs. Functional interactions between all DEGs and protein-protein interactions (PPIs) between intersection DEGs were analyzed using ReactomeFIViz and STRING, respectively, and networks were visualized. Known CRC-related genes were down-loaded from Comparative Toxicogenomics Database and mapped to PPI network. Results: Totally, 851, 760, 729, and 878 DEGs were found between control and CRC stage I, II, III, and IV, respectively. Taken together, 1235 DEGs were found, as well as 128 up-regulated intersection DEGs, 365 down-regulated intersection DEGs, and 0 contra-regulated DEG. A functional interaction network of all DEGs and a PPI network of intersection DEGs were constructed, in which CDC20, PTTG1, and MAD2L1 interacted with BUB1B; UGT2B17 interacted with ADH1B; MCM7 interacted with MCM2. BUB1B, ADH1B, and MCM2 were known CRC-related genes. Gradually upregulated expressions of CDC20, PTTG1, MAD2L1, UGT2B17, and MCM7 in stage I, II, III, and IV CRC were confirmed by using quantitative PCR. Besides, up-regulated intersection DEGs enriched in pathways about Cell cycle, DNA replication, and p53 signaling. Conclusion: CDC20, PTTG1, MAD2L1, UGT2B17, and MCM7 might be CRC stage-related genes. Highlights: 128 genes were commonly up-regulated in the 4 stages of colorectal cancer (CRC). 365 genes were commonly down-regulated in the 4 stages of CRC. CDC20, PTTG1, MAD2L1, UGT2B17, and MCM7 might be CRC stage-related genes. Above genes might promote the development of all stages of CRC.