A mechanism study on toxicity of graphene oxide to Daphnia magna: Direct link between bioaccumulation and oxidative stress. (March 2018)

• Record Type: Journal Article
• Authors: Lv, Xiaohui; Yang, Yao; Tao, Yi; Jiang, Yuelu; Chen, Baiyang; Zhu, Xiaoshan; Cai, Zhonghua; Li, Bing
• Is Part Of: Environmental pollution. Volume 234(2018)
• Page Start: 953
• Page End: 959
• Publication Date: 2018-03
• Subjects: Graphene oxide -- Daphnia magna -- Acute toxicity -- Oxidative stress -- Bioaccumulation; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2017.12.034
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Graphene oxide (GO) possesses versatile applicability and high hydrophilicity, thus may have frequent contact with aquatic organisms. However, the ecological risks of GO in aquatic ecosystems remain largely unexplored currently. This study evaluated the comprehensive toxicological effects of GO on Daphnia magna, a key species in fresh water ecosystem. The results revealed nonsevere acute toxicities, including immobility (72 h EC50 : 44.3 mg/L) and mortality (72 h LC50 : 45.4 mg/L), of GO on D. magna . To understand the underlying mechanism of GO exposure, changes in superoxide dismutase (SOD) and lipid peroxidation (LPO) of D. magna exposed to GO were correlated, which revealed elevated GO-mediated oxidative stress and damages, especially in the long-time and high-dose exposure groups. The observations of in vivo fluorescence labeled with 2′, 7′-dichlorofluorescin further demonstrated that reactive oxygen species were concentrated in daphnia guts, which corresponded with the high bioaccumulation level (5 mg/L, 24 h body burden: 107.9 g/kg) of GO in daphnia guts. However, depuration of GO from daphnia was not difficult. Daphnia almost released all GO within 24 h after it was transferred to clean water. These results hence suggest that GO could accumulate and induce significant oxidative stress in the gut of D. magna, while D. daphnia can also relieve the acute toxicity by depurating GO. Graphical abstract: Highlights: Graphene oxide (GO) can be quickly uptaken and easily depurated by D. magna. GO induces nonsevere acute toxicity in D. magna. GO mediates the production of oxidative stress in D. magna. The GO-induced oxidative stress coincides with its accumulation in daphnia guts. Abstract : This study investigated the accumulation of graphene oxide (GO) in Daphnia magna and the oxidative stress and acute toxicity induced by GO.