Influence of ozonolysis time during sugarcane pretreatment: Effects on the fiber and enzymatic saccharification. (January 2017)

• Record Type: Journal Article
• Authors: Perrone, Olavo Micali; Rossi, Jessika Souza; Moretti, Marcia Maria de Souza; Nunes, Christiane da Costa Carreira; Bordignon, Sidnei Emilio; Gomes, Eleni; Da-Silva, Roberto; Boscolo, Mauricio
• Is Part Of: Bioresource technology. Volume 224(2017)
• Page Start: 733
• Page End: 737
• Publication Date: 2017-01
• Subjects: Ozonolysis -- Pretreatment -- Sugarcane bagasse -- Enzymatic hydrolysis -- Cellulosic ethanol; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.11.043
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Secondary cell wall is the structure most affected by ozonolysis treatment. High yield conversion of cellulose using OBU treatment. Fiber changes during increase in ozonolysis treatment time were observed. SEM and TEM techniques demonstrated changes in fiber cells after ozonolysis treatment. Abstract: Modifications in sugarcane bagasse (SCB) from ozonolysis (O) NaOH (B) and ultrasound (U) (OBU) treatment for cellulosic ethanol production by enzymatic hydrolysis, were evaluated when increasing the exposure time of SCB to ozone. The lignin, cellulose, and hemicellulose after treatment were quantified: lignin removal and a consequent increase in cellulose content were shown using an infrared spectroscopic technique (ATR-FTIR) and chemical characterization. X-ray diffraction analysis (XRD) proved that OBU treatment does not affect the crystalline cellulose portion and electron microscopy techniques established that the fiber region most affected by the OBU treatment was the secondary cell wall, where the greatest lignin content is located. For OBU-60 treatment the lignin content was reduced and consequently there was a significant increase in cellulose content. After enzymatic hydrolysis, this pretreated SCB released 418 mg glucose/g, corresponding to six times more than untreated SCB and a yield of 93% of the cellulose available.