The type of dietary fat and dietary energy restriction affects the activity of the desaturases in the liver microsomes. (January 2018)

• Record Type: Journal Article
• Authors: Stawarska, Agnieszka; Białek, Agnieszka; Tokarz, Andrzej
• Is Part Of: Prostaglandins, leukotrienes, and essential fatty acids. Volume 128(2018)
• Page Start: 62
• Page End: 66
• Publication Date: 2018-01
• Subjects: ALA α-linolenic acid -- AA Arachidonic acid -- CLA Conjugated linoleic acid -- D5D Δ5-desaturase -- D6D Δ6-desaturase -- DER dietary energy restriction -- DGLA dihomo-γ-linolenic acid -- DHA Docosahexaenoic acid -- EPA Eicosapentaenoic acid -- FAME Fatty acid methyl ester -- GC gas chromatography -- HPLC high-performance liquid chromatography -- LA Linoleic acid -- OL Oleic acid -- RA Rumenic acid; Dietary energy restriction -- Oils -- Arachidonic acid -- ∆6-desaturase -- ∆5-desaturase; Lipids -- Periodicals; Unsaturated fatty acids -- Periodicals; Prostaglandins -- Periodicals; Leukotrienes -- Periodicals; Fatty Acids, Unsaturated -- Periodicals; Acides gras insaturés -- Périodiques; Prostaglandines -- Périodiques; Leucotriènes -- Périodiques; Lipides -- Périodiques; 612.01577
• DOI: 10.1016/j.plefa.2017.12.001
• Languages: English
• ISSNs: 0952-3278

Abstract

Abstract: The aim of present study was to investigate the effect of different dietary oils and the dietary energy restriction on the activity of enzymes participating in the process of arachidonic acid synthesis and on fatty acid profile in serum. It was also evaluated how diet modification affects the weight of animals and weight of the specific organs: liver, kidney and spleen. Wistar male rats were divided into 6 groups according to the diet fed (control, sunflower oil, olive oil, rapeseed oil, fish oil and a group of dietary energy restriction - DER group). The enzyme activities were established indirectly in liver microsomes. To this aim the method of high performance liquid chromatography with UV/VIS detection was used. In addition, the indices of ∆ 6 -desaturase (D6D) and ∆ 5 -desaturase (D5D) were determined. Significant differences in the concentrations of fatty acids and enzyme activity were observed. The results concerning desaturases show the negative correlation between n-3 polyunsaturated fatty acids intake and enzymes activity. The highest D6D activity was observed in microsomes obtained from sunflower oil fed rats and the lowest D6D activity was in the DER group. D5D index did not differ much depending on the diet. Among groups supplemented with oils the higher mean values of the weight of liver were observed in the group supplemented with rapeseed oil. Consumption of diets supplemented with edible oils of different fatty acid profile influence both serum fatty acid composition and the activity of ∆ 6 - and Δ 5 -desaturase. Highlights: Diet affects the fatty acids profile, the activity of desaturases, weight of animals and weight of specific organs. Diet modification affects the weight of animals and weight of the specific organs: liver, kidney, spleen. Restrictive diet significantly inhibits enzymes activity, which may influence fatty acid conversion and eicosanoid synthesis.