Effect of plant-based carbon sources on denitrifying microorganisms in a vertical flow constructed wetland. (January 2017)

• Record Type: Journal Article
• Authors: Fu, Guiping; Huangshen, Linkun; Guo, Zhipeng; Zhou, Qiaohong; Wu, Zhenbin
• Is Part Of: Bioresource technology. Volume 224(2017)
• Page Start: 214
• Page End: 221
• Publication Date: 2017-01
• Subjects: Gene abundance -- Nitrogen removal pathway -- Partial nitrification-denitrification -- Plant-based carbon source -- Vertical flow constructed wetland; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.11.007
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Combined plant-based carbon source could improve the N removal efficiency of VFCWs. Effects of plant-based carbon sources on N removal genes were estimated. Partial nitrification-denitrification and classic denitrification found in VFCWs. Abstract: The effects of supplementing plant-based carbon sources, fermented tissues of Arundo donax and Pontederia cordata, and a combination of the two plants, on the nitrogen removal efficiency and microbial composition in a vertical flow constructed wetland (VFCW) were examined. The results showed that the addition of the composite carbon source produced the highest removal efficiencies of NH4 + -N 91.5%, NO3 − -N 94.5% and TN 92.8% in VFCW. The detected abundance of amoA, nirS, and nxrA genes indicated that ammonia oxidation bacteria and denitrifying bacteria were more abundant than the nitrite oxidation bacteria. Furthermore, the addition of the composite carbon source significantly promoted the growth of the denitrifying bacteria in VFCW. The results indicated that supplementing the system with plant-based carbon sources achieved partial nitrification and denitrification, as well as classic denitrification in VFCWs. The study suggested that multiple nitrogen removal pathways were required to feasibly and efficiently remove nitrogen.