Nanoreactors based on DNAzyme-functionalized magnetic nanoparticles activated by magnetic field. Issue 3 (3rd January 2018)

• Record Type: Journal Article
• Authors: Bakshi, Saira F.; Guz, Nataliia; Zakharchenko, Andrey; Deng, Han; Tumanov, Alexei V.; Woodworth, Craig D.; Minko, Sergiy; Kolpashchikov, Dmitry M.; Katz, Evgeny
• Is Part Of: Nanoscale. Volume 10:Issue 3(2018)
• Page Start: 1356
• Page End: 1365
• Publication Date: 2018-01-03
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/c7nr08581h
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : A new biomimetic nanoreactor for analysis of mRNA cancer biomarkers is presented based on DNAzyme bound to superparamagnetic nanoparticles. Abstract : A new biomimetic nanoreactor design, MaBiDz, is presented based on a copolymer brush in combination with superparamagnetic nanoparticles. This cellular nanoreactor features two species of magnetic particles, each functionalized with two components of a binary deoxyribozyme system. In the presence of a target mRNA analyte and a magnetic field, the nanoreactor is assembled to form a biocompartment enclosed by the polymeric brush that enables catalytic function of the binary deoxyribozyme with enhanced kinetics. MaBiDz was demonstrated here as a cellular sensor for rapid detection and imaging of a target mRNA biomarker for metastatic breast cancer, and its function shows potential to be expanded as a biomimetic organelle that can downregulate the activity of a target mRNA biomarker.