Isolation, purification, structural characterization and immunostimulatory activity of water-soluble polysaccharides from Lepidium meyenii. (March 2018)

• Record Type: Journal Article
• Authors: Zha, Zhengqi; Wang, Su-Yan; Chu, Weihua; Lv, Yang; Kan, Hongjin; Chen, Qiuli; Zhong, Lili; Yue, Long; Xiao, Jinna; Wang, Ying; Yin, Hongping
• Is Part Of: Phytochemistry. Volume 147(2018)
• Page Start: 184
• Page End: 193
• Publication Date: 2018-03
• Subjects: Lepidium meyenii Walp -- Polysaccharide -- Macrophage RAW264.7 -- Toll-like receptor (TLR) -- Nuclear factor-κB; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2018.01.006
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: A water-soluble polysaccharide LMP-1 was isolated and purified by ion-exchange chromatography from maca ( Lepidium meyenii Walp.). LMP-1 has a molecular weight of 1.01 × 10 4 Da, and is composed of glucose and arabinose with a molar ratio of 7.03:1.08. Methylation and the 1D and 2D NMR spectroscopy of LMP-1 revealed that it is mainly composed of →4)-α-D -Glc p -(1→, →6)-α-D -Glc p -(1→, →3)-α-D -Glc p -(1→, and β-D -Ara f -(1→, with branching at O-6 of →4, 6)-α-D -Glc p -(1 → . LMP-1 showed up-regulation of Toll-like receptor 4 (TLR4) and Toll-like receptor 2 (TLR2). The upstream proteins of Toll-like receptors (TLRs) (CD14 and MD2) and mRNA level of IL-1β also increased. Increased transcription factor nuclear factor-kappa B (NF-κB) p65 was found in the nuclei and cytoplasm in LMP-1-treated RAW264.7 macrophages. These results indicated that LMP-1 activated RAW264.7 macrophages and elicited immunostimulatory activities via the TLRs/NF-κB signalling pathway. Graphical abstract: Highlights: A previously undescribed polysaccharide (LMP-1) was purified from Lepidium meyenii Walp. The structural features of LMP-1 of Lepidium meyenii Walp. were investigated. LMP-1 activated RAW264.7 macrophages and elicited immunostimulatory activities via the TLRs/NF-κB pathway.