Differentiation analysis of boron isotopic fractionation in different forms within plant organ samples. (March 2018)

• Record Type: Journal Article
• Authors: Sun, Aide; Xu, Qingcai; Wei, Gangjian; Zhu, Huayu; Chen, Xuefei
• Is Part Of: Phytochemistry. Volume 147(2018)
• Page Start: 9
• Page End: 13
• Publication Date: 2018-03
• Subjects: Boron -- Fractionation factor -- Isotope composition -- Multi-collector inductively coupled plasma mass spectrometry -- Plant; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2017.12.012
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: As a critical micronutrient, boron (B) plays an important role in plant growth and embryonic development. To further understand the effects of B uptake, transportation and isotopic fractionation, the contents and isotopic compositions of hydro-soluble B in the sap and structural B fixed in the cell within individual plant tissues were investigated. The B isotope ratio was determined by multi-collector inductively coupled plasma mass spectrometry. The δ 11 B values in hydro-soluble and structural B in the investigated plant samples ranged from −1.57‰ to +11.30‰ and from +6.57‰ to +16.64‰, respectively. Different fractionation factors of the B isotopes, in the range of 0.9954–1.0150, were observed in these samples, indicating that in most plant tissues, the heavy isotope ( 11 B) was preferentially enriched in structural B, which was fixed into the cell. However, there was a reversal in the fractionation of B isotopic compositions in the fruit samples compared with the other plant tissue samples. It is more powerful to examine the molecular mechanisms of B transport, uptake and utilization than the use of limited plant organ samples containing a mixture of hydro-soluble and structural B within different intra-plant compartments and in inter-plant interactions. These isotopic shifts, which may be used as important isotopic indicators, contribute to the surface processes interactions in the plant–soil system and the knowledge of the molecular mechanisms of B in the uptake and absorption by different plant species in nature. Graphical abstract: Highlights: Isotopes of B show different ratios in different metabolite pools. Analysis of two isotopes of B helps understand B distribution in plant nutrition. Two different fractionation trends of B isotope are seen in the plant studies.