A novel screening method to identify air pollution by genotoxic compounds. (March 2018)

• Record Type: Journal Article
• Authors: Baráková, Daniela; Sharma, Anežka; Chropeňová, Mária; Čupr, Pavel
• Is Part Of: Environmental pollution. Volume 234(2018)
• Page Start: 473
• Page End: 479
• Publication Date: 2018-03
• Subjects: Genotoxicity -- Air pollution monitoring -- Coniferous needles -- SOS chromotest -- Persistent organic pollutants; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2017.11.061
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Genotoxic compounds, as common contaminants of the air environment, are of interest in air pollution monitoring. There are several methods to determine the level of these contaminants in different localities, many of which may be difficult to access with the use of conventional active and passive samplers. In the present study, the needles Pinus mugo Turra and Picea abies were used to monitor sampling localities in Austria, Slovakia, and the Czech Republic. Needles were extracted and chemical analysis and the genotoxicity bioassay SOS chromotest were used to obtain complex information about the chemical mixture of pollutants present and their genotoxic effects. The SOS chromotest method was optimized by using a CPRG chromogenic substrate to reduce the false positive genotoxic effect of needle extracts. Pinus mugo Turra and Picea abies were identified as suitable passive sampling matrices for long-term air monitoring using the same plants sampled at the same time. The presented study brings an innovative method for the fast screening and identification of localities loaded by genotoxic active air contaminants. Graphical abstract: Highlights: A new screening approach to identify the genotoxic potential of air pollution. The first use of needles as a sampling matrix to monitor genotoxic air pollutants. Method optimization of the SOS chromotest to measure genotoxic potential in needles. The combination of chemical analysis and bioassay on the same sample. Abstract : Extracts from coniferous needle samples can be used to assess the genotoxic potency of contaminated air.