N-acetylcysteine leads to greater ovarian protection than enoxaparin sodium in a rat ovarian torsion model. Issue 1 (July 2016)

• Record Type: Journal Article
• Authors: Sahin Ersoy, Gulcin; Eken, Meryem; Tal, Reshef; Oztekin, Deniz; Devranoglu, Belgin; Isik Kaygusuz, Ecmel; Cevik, Ozge
• Is Part Of: Reproductive biomedicine online. Volume 33:Issue 1(2016)
• Page Start: 93
• Page End: 101
• Publication Date: 2016-07
• Subjects: anti-Müllerian hormone -- enoxaparin sodium -- N-acetylcysteine -- ovarian ischaemia-reperfusion -- ovarian reserve -- torsion; Human reproductive technology -- Periodicals; Human embryo -- Periodicals; Reproduction -- Periodicals; 616.692
• DOI: 10.1016/j.rbmo.2016.03.009
• Languages: English
• ISSNs: 1472-6483

Abstract

Abstract: This study evaluated the effects of N-acetylcysteine (NAC) and enoxaparin on ovarian tissue preservation, ovarian reserve and oxidative damage following ovarian torsion/detorsion injury. Rats were divided into four groups ( n = 6/group): control; ischaemia/reperfusion (I/R); I/R + NAC; I/R + enoxaparin. Twenty-four hours after detorsion, ovarian tissues were collected for histopathological analysis and measurement of tissue 8-OHdG, GSH, MDA, MPO and SOD concentrations, as well as pre- and post-operative circulating AMH concentrations. Administration of NAC resulted in more pre-antral follicles compared with enoxaparin treatment and haemorrhage and follicle cell degeneration were more pronounced in I/R + enoxaparin group than I/R + NAC group. Both NAC and enoxaparin led to a significant reduction in ovarian tissue 8-OHdG ( P = 0.004 and P = 0.01, respectively) and MPO ( P = 0.013 and P = 0.023, respectively) concentrations compared with I/R group, indicating a protective effect against I/R oxidative damage. Only NAC-treated animals showed a significant increase in GSH and SOD concentrations and decrease in MDA concentrations compared with I/R group ( P = 0.007, P = 0.024 and P = 0.026, respectively). These results indicate that NAC is more effective than enoxaparin in minimizing ovarian damage and preserving ovarian reserve following ovarian torsion.