Characterization of antigen-presenting cells from the porcine respiratory system. (June 2015)

• Record Type: Journal Article
• Authors: López-Robles, Guadalupe; Silva-Campa, Erika; Burgara-Estrella, Alexel; Hernández, Jesús
• Is Part Of: Research in veterinary science. Volume 100(2015)
• Page Start: 80
• Page End: 87
• Publication Date: 2015-06
• Subjects: Antigen-presenting cells -- Dendritic cells -- Porcine -- Respiratory system -- Macrophages; Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2015.04.009
• Languages: English
• ISSNs: 0034-5288

Abstract

Highlights: Antigen-presenting cells are strategically placed in all anatomic sites. This study evaluated properties of respiratory antigen-presenting cells. Cells from lymph node and lung resemble specialized antigen-presenting cells in contrast to alveolar macrophages Abstract: Antigen-presenting cells (APCs) are strategically placed in all anatomic sites with high antigen exposure such as the respiratory system. The aim of this study was to evaluate phenotypic and functional properties of APCs from the lung (L-Cs), mediastinal lymph node (LN-Cs) and bronchoalveolar lavage cells (BAL-Cs). The APCs were first analyzed based on forward scatter and side scatter profiles and the selection of MHC-II high CD172a + cells (referred to as APCs); then the expression of CD1a, CD163, CD206, CD16 and CD11R3 was evaluated in the APCs. The results showed that CD1a, CD163 and CD206 were differentially expressed among L-Cs, LN-Cs and BAL-Cs, suggesting the phenotype MHC-II high CD172a + CD1a low/- CD163 low CD206 - for L-Cs and MHC-II high CD172a + CD1a + CD163 low/- CD206 + for LN-Cs. BAL-Cs were MHC-II high CD172a + CD1a - CD163 high CD206 +/- . The functional characteristics of L-Cs and LN-Cs were different from those of BAL-Cs, confirming that L-Cs and LN-Cs resemble specialized APCs. In conclusion, we present the characterization of APCs from L-Cs, LN-Cs and BAL-Cs of the porcine respiratory system.