Antimicrobial activity of LFchimera synthetic peptide against plant pathogenic bacteria. (14th December 2017)

• Record Type: Journal Article
• Authors: Chahardoli, Mahmood; Fazeli, Arash; Ghabooli, Mehdi
• Is Part Of: Archives of phytopathology and plant protection. Volume 50:Number 19/20(2017)
• Page Start: 1008
• Page End: 1018
• Publication Date: 2017-12-14
• Subjects: LFchimera -- antimicrobial activity -- plant pathogenic bacteria -- agar disc diffusion -- MIC; Plants, Protection of -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1080/03235408.2017.1411173
• Languages: English
• ISSNs: 0323-5408

Abstract

Abstract: The present study was designed to evaluate potential antibacterial activities of synthetic LFchimera against five plant pathogenic bacteria such as Ralstonia solanacearum, Erwinia amylovora, Xanthomonas campestris, Pseudomonas syringae and Pectobacterium carotovorum . The agar disc-diffusion method with different concentrations (0.2, 0.4, 0.6 and 0.8 μM) of peptide was used to study the antibacterial activity of LFchimera against bacteria. The Minimum Inhibitory Concentration (MIC) of the LFchimera peptide were tested using serial dilution method at concentration ranging from 0 to 10 μM. The Results from agar disc-diffusion method revealed that LFchimera was effective against all bacterial strain in a dose-dependent manner. LFchimera showed highest activity in 0.8 μM which was significant compared to the standard antibiotic. LFchimera pepetide showed low MIC values (4 μM) against all tested bacteria. LFchimera peptide was found to show antibacterial activity against important phytopathogenic bacteria and can improve the potential of an antimicrobial peptide in plant disease management.