Metabolic effects of Hedyotis diffusa on rats bearing Walker 256 tumor revealed by NMR‐based metabolomics. (12th September 2017)

• Record Type: Journal Article
• Authors: Wang, Zhiyong; Gao, Kuo; Xu, Can; Gao, Jian; Yan, Yujing; Wang, Yingfeng; Li, Zhongfeng; Chen, Jianxin
• Is Part Of: Magnetic resonance in chemistry. Volume 56:Number 1(2018)
• Page Start: 5
• Page End: 17
• Publication Date: 2017-09-12
• Subjects: 1H NMR -- biomarkers -- Hedyotis diffusa -- metabolomics -- Walker 256 tumor; Nuclear magnetic resonance spectroscopy -- Periodicals; Chemistry, Organic -- Periodicals; Magnetic resonance -- Periodicals; 538.36
• DOI: 10.1002/mrc.4658
• Languages: English
• ISSNs: 0749-1581

Abstract

Abstract: Hedyotis diffusa, a traditional Chinese herbal medicine, is widely used for oncotherapy and shows a positive effect in the clinical treatment. But its mechanism of anticancer activities is complicated and unclear. This study was undertaken to assess the therapeutic effects and reveal detailed mechanisms of H . diffusa for oncotherapy. A Walker 256 tumor‐bearing rat model was established, and metabolomic profiles of plasma and urine were obtained from 1 H NMR technique. Multivariate statistical analysis methods were used to characterize the discriminating metabolites between control (C), Walker 256 tumor‐bearing rats model (M), and H . diffusa treatment (H) groups. Finally, 13 and 10 metabolomic biomarkers in urine and plasma samples were further identified as characteristic metabolites in M group, whereas H group showed a partial metabolic balance recovered, such as ornithine, N ‐acetyl‐l ‐aspartate, l ‐aspartate, and creatinine in urine samples, and acetate, lactate, choline, l ‐glutamine, and 3‐hydroxybutyrate in plasma samples. On the basis of the methods above, we hypothesized H . diffusa treatment reduced the injury caused by Walker 256 tumor and maintained a metabolic balance. Our study demonstrated that this method provided new insights into metabolic alterations in tumor‐bearing biosystems and researching on the effects of H . diffusa on the endogenous metabolism in tumor‐bearing rats. Abstract : A walker 256 tumor‐bearing rat model was established, and metabolomic profiles of plasma and urine obtained from 1 H NMR technique combined with time‐course metabolites analysis were carried out to assess the therapeutic effects and reveal detailed mechanisms of Hedyotis diffusa for oncotherapy. We hypothesized Hedyotis diffusa treatment reduced the injury caused by Walker 256 tumor and maintained a metabolic balance, and such effect might result from amino acid metabolism, energy metabolism, and lipid metabolism.