The rice RCN11 gene encodes β1, 2-xylosyltransferase and is required for plant responses to abiotic stresses and phytohormones. (July 2015)

• Record Type: Journal Article
• Authors: Takano, Sho; Matsuda, Shuichi; Funabiki, Atsushi; Furukawa, Jun-ichi; Yamauchi, Takaki; Tokuji, Yoshihiko; Nakazono, Mikio; Shinohara, Yasuro; Takamure, Itsuro; Kato, Kiyoaki
• Is Part Of: Plant science. Volume 236(2015:Jul.)
• Page Start: 75
• Page End: 88
• Publication Date: 2015-07
• Subjects: β1, 2-Xylosyltransferase -- N-Glycan -- Rice -- Low temperature -- Abiotic stresses -- Root aerenchyma formation; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2015.03.022
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Rice REDUCED CULM NUMBER 11 encodes β1, 2-xylosyltransferase. RCN11 is involved in plant growth under low and high temperature conditions. RCN11 mediates ABA sensitivity during seed germination. RCN11 expression is regulated by multiple phytohormones. Abstract: Seed germination rates and plant development and growth under abiotic stress are important aspects of crop productivity. Here, our characterization of the rice ( Oryza sativa L.) mutant reduced culm number11 ( rcn11 ) showed that RCN11 controls growth of plants exposed to abnormal temperature, salinity and drought conditions. RCN11 also mediates root aerenchyma formation under oxygen-deficient conditions and ABA sensitivity during seed germination. Molecular studies showed that the rcn11 mutation resulted from a 966-bp deletion that caused loss of function of β1, 2-xylosyltransferase (OsXylT). This enzyme is located in the Golgi apparatus where it catalyzes the transfer of xylose from UDP-xylose to the core β-linked mannose of N -glycans. RCN11 / OsXylT promoter activity was observed in the basal part of the shoot containing the shoot and axillary meristems and in the base of crown roots. The level of RCN11 / OsXylT expression was regulated by multiple phytohormones and various abiotic stresses suggesting that plant specific N -glycosylation is regulated by multiple signals in rice plants. The present study is the first to demonstrate that rice β1, 2-linked xylose residues on N -glycans are critical for seed germination and plant development and growth under conditions of abiotic stress.