A two-color fluorescence enhanced dot-blot assay for revealing co-operative expression of chemokine receptors in cells. Issue 7 (8th January 2018)

• Record Type: Journal Article
• Authors: Khan, Naseer Ullah; He, Hua; Wang, Xiaojuan; Ge, Baosheng; Wang, Qian; Liu, Xu; Lao, Jun; Wang, Yanan; Li, Jiqiang; Wang, Zhencai; Zhou, Shitan; Huang, Fang
• Is Part Of: Chemical communications. Volume 54:Issue 7(2018)
• Page Start: 778
• Page End: 781
• Publication Date: 2018-01-08
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc08167g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A highly sensitive two-color dot-blot assay has been developed to simultaneously detect co-operative expression of chemokine receptors in cells. Abstract : An ultra-high fluorescence enhancement for two dyes on photonic crystal films was achieved to construct a two-color immuno-dot blot assay. This assay was demonstrated to simultaneously detect chemokine receptor co-expressed in cancer cells and reveal their co-operative and subtle changes after binding with respective ligands and drugs.