25‐Hydroxy vitamin D suppresses hepatitis C virus replication and contributes to rapid virological response of treatment efficacy. Issue 13 (24th March 2017)

• Record Type: Journal Article
• Authors: Huang, Jee‐Fu; Ko, Yu‐Min; Huang, Chung‐Feng; Yeh, Ming‐Lun; Dai, Chia‐Yen; Hsieh, Meng‐Hsuan; Huang, Ching‐I; Yang, Hua‐Ling; Wang, Shu‐Chi; Lin, Zu‐Yau; Chen, Shinn‐Chern; Yu, Ming‐Lung; Chuang, Wan‐Long
• Is Part Of: Hepatology research. Volume 47:Issue 13(2017)
• Page Start: 1383
• Page End: 1389
• Publication Date: 2017-03-24
• Subjects: 25‐hydroxy vitamin D -- hepatitis C virus -- replicon -- viral kinetics; Liver -- Diseases -- Periodicals; Liver Diseases -- Periodicals; Foie -- Maladies -- Périodiques; 616.362
• DOI: 10.1111/hepr.12878
• Languages: English
• ISSNs: 1386-6346

Abstract

Abstract : Aim: 25‐Hydroxy vitamin D (Vit D) plays a role in treatment outcomes in chronic hepatitis C virus (HCV) infection. We aimed to clarify whether HCV replication is inhibited by Vit D in HCV replicon cells. Clinical implication was assessed for rapid virological response (RVR) and sustained virological response (SVR) among those patients receiving antiviral therapy. Methods: Cell survival and viral loads were observed in Con1 (genotype 1b) and J6/JFH (genotype 2a) cells treated with different doses of Vit D. Three groups of patients with different treatment responses were recruited to assess their Vit D levels: group A, RVR−/SVR−; group B, RVR+/SVR−; and group C, RVR+/SVR+. Results: The viral load of Con1 cells decreased by 69%, 80%, and 86% following treatment with 1 μM, 5 μM, and 10 μM Vit D, respectively ( P < 0.0001). In J6/JFH cells, it decreased by 12%, 55%, and 80.5% following treatment with 1 μM, 5 μM, and 10 μM Vit D, respectively ( P < 0.0001). There was a significant increase of Vit D between chronic hepatitis C groups, ranging from 4.4 ± 5.6 ng/mL in group A ( n = 44), to 17.2 ± 11.6 ng/mL in group B ( n = 44), and 32.5 ± 37.5 ng/mL of group C ( n = 44) ( P < 0.001). Advanced fibrosis (odds ratio = 0.13, 95% confidence interval = 0.04–0.41, P < 0.001) and Vit D deficiency (<10 ng/mL) (odds ratio = 0.11, 95% confidence interval = 0.03–0.43, P = 0.001) were predictive of SVR in the multivariate regression analysis. Conclusion: Vitamin D decreases HCV replication and also contributes to early treatment viral kinetics.