A cytosolic sensor, PmDDX41, mediates antiviral immune response in black tiger shrimp Penaeus monodon. (April 2018)

• Record Type: Journal Article
• Authors: Soponpong, Suthinee; Amparyup, Piti; Tassanakajon, Anchalee
• Is Part Of: Developmental and comparative immunology. Volume 81(2018)
• Page Start: 291
• Page End: 302
• Publication Date: 2018-04
• Subjects: DDX41 -- DNA-sensing pathway -- Shrimp immunity -- Antiviral response -- White spot syndrome virus; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2017.12.013
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: DEAD (Asp-Glu-Ala-Asp)-box polypeptide 41 (DDX41), a receptor belonging to the DExD family, has recently been identified as an intracellular DNA sensor in vertebrates. Here, we report on the identification and functional characterization of Pm DDX41, the first cytosolic DNA sensor in shrimp. By searching a Penaeus monodon expressed sequence tag (EST) database (http://pmonodon.biotec.or.th ), three cDNA fragments exhibiting similarity to DDX41 in various species were identified and assembled, resulting in a complete open reading frame of Pm DDX41 that contains 1863-bp and encodes a putative protein of 620 amino acids. Pm DDX41 shares 83% and 79% similarity to DDX41 homolog from the bee Apis florea and fruit fly Drosophila melanogaster, respectivel y and contains three conserved domains in the protein: DEADc domain, HELICc domain, and zinc finger domain. The transcript of Pm DDX41 was detected in all tested tissues and was up-regulated upon infection with a DNA virus, white spot syndrome virus (WSSV). However, Pm DDX41 mRNA expression was not significantly changed and down-regulated in response to a bacterium, Vibrio harveyi, or an RNA virus, yellow head virus (YHV), respectively, compared with the control phosphate-buffered saline-injected shrimp. Furthermore, the suppression of Pm DDX41 by dsRNA-mediated gene silencing resulted in more rapid death of WSSV-infected shrimp and a significant decrease in the mRNA expression levels of several immune-related genes ( Pm IKKβ, Pm IKKɛ, Pm Relish, Pm Cactus, Pm Dorsal, Pm PEN3, Pm PEN5, and ALF Pm 6). These results suggest that Pm DDX41 is involved in the antiviral response, probably via a DNA-sensing pathway that is triggered through the IκB kinase complex and leads to the activation of several immune-related genes. Highlights: Pm DDX41 cDNA was identified in shrimp. Pm DDX41 transcript was up-regulated in hemocytes after WSSV challenge. Pm DDX41-knockdown shrimp showed a decreased survival rate after WSSV infection. Pm DDX41 is involved in the antiviral response, probably via a DNA-sensing pathway.