Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test. (November 2015)

• Record Type: Journal Article
• Authors: Prod'hom, Guy; Durussel, Christian; Blanc, Dominique; Croxatto, Antony; Greub, Gilbert
• Is Part Of: New microbes and new infections. Volume 8(2015)
• Page Start: 1
• Page End: 3
• Publication Date: 2015-11
• Subjects: Ammonium chloride -- blood culture -- cephalosporins -- chromogenic cephalosporin -- β-lactamase -- rapid testing; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; Microbiology -- Periodicals; Communicable Diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1016/j.nmni.2015.05.007
• Languages: English
• ISSNs: 2052-2975

Abstract

Abstract: Bacterial pellets from Enterobacteriaceae positive blood cultures prepared using ammonium chloride were tested for rapid detection of β-lactamase using the commercial βLACTA test and read after 30 minutes. During 7 months, 137 bacterial pellets were tested prospectively. βLACTA test exhibited a sensitivity of 75% and a specificity of 100% for the detection of third-generation cephalosporin resistance. False negative tests were mainly observed with hyperproduced chromosomal or plasmid-borne AmpC.