1H NMR Based Metabolomics Studies of the Toxicity of Titanium Dioxide Nanoparticles in Zebrafish (Danio rerio). (14th December 2017)

• Record Type: Journal Article
• Authors: Raja, Ganesan; Kim, Siwon; Yoon, Dahye; Yoon, Changshin; Kim, Suhkmann
• Is Part Of: Bulletin of the Korean Chemical Society. Volume 39:Number 1(2018)
• Page Start: 33
• Page End: 39
• Publication Date: 2017-12-14
• Subjects: 1H NMR -- Metabolomics -- Mesoporous carbon nanoparticles -- Cytotoxicity -- Zebrafish; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/bkcs.11336
• Languages: English
• ISSNs: 0253-2964

Abstract

Abstract : Titanium dioxide nanoparticles (TiO2 ‐NPs) are widely used in foods, cosmetics, and pharmaceuticals. In the present study, male and female zebrafish ( Danio rerio ) were exposed to sub‐lethal concentrations of TiO2 ‐NPs dosed at 0.1 mg/L (low) and 5.0 mg/L (high), respectively. 1 H–nuclear magnetic resonance (NMR) profiling combined with univariate and multivariate statistics was used to assess metabolic alterations in the TiO2 ‐NP‐treated fish. Purine metabolism—including the anabolism/catabolism of ATP, ADP, AMP, xanthine, and inosine—was significantly influenced, which may indicate some genotoxicity resulting from TiO2 ‐NP exposure. Increasing the dose and duration of TiO2 ‐NP exposure led to additional metabolic disturbances in glycerophospholipid metabolism and the Krebs cycle. Our findings demonstrate the potential applicability of using a metabolomics approach to determine underlying metabolic disturbances caused by TiO2 ‐NPs. Abstract :