Label‐free imaging of healthy and infarcted fetal sheep hearts by two‐photon microscopy. Issue 1 (2nd May 2017)

• Record Type: Journal Article
• Authors: Sorvina, Alexandra; Bader, Christie A.; Lock, Mitchell C.; Brooks, Douglas A.; Morrison, Janna L.; Plush, Sally E.
• Is Part Of: Journal of biophotonics. Volume 11:Issue 1(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-05-02
• Subjects: fetus -- myocardial infarction -- two-photon microscopy -- collagen -- metabolic activity; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201600296
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: Coronary heart disease is one of the largest causes of death worldwide, making this a significant health care issue. A critical problem for the adult human heart is that it does not undergo effective repair in response to damage, leaving patients with a poor prognosis. Unlike the adult, fetal hearts have the ability to repair after myocardial damage. Using two‐photon microscopy, we have visualised the morphological and metabolic changes following myocardial infarction in sheep fetuses, to characterise response to cardiac injury in a mammalian model. Following myocardial infarction, fetal hearts showed no significant increase in collagen deposition in the region of the infarction, when compared to either the surrounding tissue or shams. In contrast, metabolic activity (i. e. NAD(P)H and FAD) was significantly reduced in the region of myocardial infarction, when compared to either the surrounding tissue or sham hearts. For comparison, we also imaged two hearts from preadolescent sheep (sham and myocardial infarction) and showed highly ordered collagen deposition with decreased metabolic activity within the infarcted area. Therefore, two‐photon imaging had the capacity to image both morphological and metabolic changes in response to myocardial infarction and showed differences in the response with age.Picture : Two‐photon imaging of myocardial infarction (b andd ) enabled the visualisation of increased collagen (blue; Em=431 nm) and changes in other tissue autofluorescence (green; Em=489–606 nm) in fetal (a andb ) and preadolescent (c andd ) hearts, compared to shams (a andc ). The excitation wavelength was 840 nm. Scale bars: 10 μm. Abstract : Unlike the adult, fetal hearts have the ability to repair after myocardial damage. Using two‐photon microscopy, we visualised the morphological and metabolic changes following myocardial infarction in sheep fetuses and lambs. Fetal hearts showed no significant increase in collagen deposition in the infarct area, however, metabolic activity was significantly reduced. In preadolescent sheep; myocardial infarction resulted in highly ordered collagen deposition within the infarct area with decreased metabolic activity.