Front Cover: A theoretical‐experimental methodology for assessing the sensitivity of biomedical spectral imaging platforms, assays, and analysis methods (J. Biophotonics 1/2018). Issue 1 (17th January 2018)

• Record Type: Journal Article
• Authors: Leavesley, Silas J.; Sweat, Brenner; Abbott, Caitlyn; Favreau, Peter; Rich, Thomas C.
• Is Part Of: Journal of biophotonics. Volume 11:Issue 1(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-01-17
• Subjects: spectral -- spectroscopy -- microscopy -- linear unmixing -- spectral angle mapper -- constrained energy minimization -- matched filter; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201870125
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : A hyperspectral image data cube acquired from HEK‐293 cells labeled with cytoplasmic and nuclear stains: Calcein Green and NucBlu. The top view (XY plane) displays three spectrally unmixed channels for cellular autofluorescence (red), Calcein Green (green), and NucBlue (blue). The Z axis shows spectral information, from low to high wavelength. The article by Leavesley and colleagues describes an approach for calculating the sensitivity of spectral imaging assays for detecting a fluorescence signature within a mix of other signatures or autofluorescence. Further details can be found in the article by Silas J. Leavesley et al. (e201600227 ). Abstract : A hyperspectral image data cube acquired from HEK‐293 cells labeled with cytoplasmic and nuclear stains: Calcein Green and NucBlu. The top view (XY plane) displays three spectrally unmixed channels for cellular autofluorescence (red), Calcein Green (green), and NucBlue (blue). The Z axis shows spectral information, from low to high wavelength. The article by Leavesley and colleagues describes an approach for calculating the sensitivity of spectral imaging assays for detecting a fluorescence signature within a mix of other signatures or autofluorescence. Further details can be found in the article by Silas J. Leavesley et al. (e201600227 ).