The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal‐to‐zygotic transition. (10th January 2017) - Yang, Ye
Abstract: The functional role of the ubiquitin‐proteasome pathway during maternal‐to‐zygotic transition (MZT) remains to be elucidated. Here we show that the E3 ubiquitin ligase, Rnf114, is highly expressed in mouse oocytes and that knockdown of Rnf114 inhibits development beyond the two‐cell stage. To study the underlying mechanism, we identify its candidate substrates using a 9, 000‐protein microarray and validate them using an in vitro ubiquitination system. We show that five substrates could be degraded by RNF114‐mediated ubiquitination, including TAB1. Furthermore, the degradation of TAB1 in mouse early embryos is required for MZT, most likely because it activates the NF‐κB pathway. Taken together, our study uncovers that RNF114‐mediated ubiquitination and degradation of TAB1 activate the NF‐κB pathway during MZT, and thus directly link maternal clearance to early embryo development. Synopsis: The E3 ubiquitin ligase RNF114 promotes TAB1 ubiquitination and degradation, and both RNF114 and TAB1 downregulation are required for maternal‐to‐zygotic transition of mouse embryos. RNF114 is expressed in the oocyte and during early embryo development. TAB1 is ubiquitinated by RNF114 during MZT. The degradation of TAB1 activates the NF‐κB pathway essential for early embryo development. Abstract : The E3 ubiquitin ligase RNF114 promotes TAB1 ubiquitination and degradation, and both RNF114 and TAB1 downregulation are required for maternal‐to‐zygotic transition of mouse embryos.