Genetic analysis of Cheirostylis species based on microsatellite markers. Issue 3 (2nd October 2014)

• Record Type: Journal Article
• Authors: Sraphet, Supajit; Saengsri, Anuwat; Smith, Duncan R.; Triwitayakorn, Kanokporn
• Is Part Of: Plant genetic resources. Volume 13:Issue 3(2015)
• Page Start: 286
• Page End: 289
• Publication Date: 2014-10-02
• Subjects: Cheirostylis sp, -- cross-amplification, -- enriched genomic library, -- microsatellite; Plant genetics -- Periodicals; Plant breeding -- Periodicals; 632.523
• DOI: 10.1017/S1479262114000914
• Languages: English
• ISSNs: 1479-2621

Abstract

Abstract : Microsatellite markers specific to Cheirostylis yunnanensis Rolfe were developed using an enriched genomic DNA library technique. The library was constructed using (AG)20 and (CAG)20 oligonucleotide repeats. A total of 48 primer pairs were designed and tested with 48 C. yunnanensis Rolfe samples, resulting in 11 polymorphic loci. The number of alleles per locus ranged from 2 to 12, with an average of six alleles. The observed and expected heterozygosity ranged from 0.0426 to 0.8085 and 0.0421 to 0.9078, respectively. Of the 11 polymorphic loci, three showed a significant deviation from Hardy–Weinberg equilibrium and one exhibited linkage disequilibrium. Cross-species amplification was tested with five samples of Cheirostylis of unknown species resulting in eight loci that could be amplified, with the number of alleles ranging from one to two. The microsatellite markers developed in this study will be useful for the genetic analysis of C. yunnanensis in order to differentiate species as well as to establish a conservation plan for this species.