Antihyperlipidemic and hepatoprotective activities of polysaccharide fraction from Cyclocarya paliurus in high-fat emulsion-induced hyperlipidaemic mice. (1st March 2018)

• Record Type: Journal Article
• Authors: Yang, Zhanwei; Wang, Jin; Li, Jingen; Xiong, Lei; Chen, Hui; Liu, Xin; Wang, Ning; Ouyang, Kehui; Wang, Wenjun
• Is Part Of: Carbohydrate polymers. Volume 183(2018)
• Page Start: 11
• Page End: 20
• Publication Date: 2018-03-01
• Subjects: CPP 2 Cyclocarya paliurus polysaccharide fraction eluted with 0.2% NaCl aqueous solution -- Mw weight-average molecular weight -- TC total cholesterol -- TG triacylglycerols -- HDL-C high-density lipoprotein cholesterol -- LDL-C low-density lipoprotein cholesterol -- SOD superoxide dismutase -- T-AOC total antioxidant capability -- GSH-PX glutathione peroxidase -- MDA malondialdehyde -- LPO lipid peroxide -- GPT glutamate pyruvate transaminase -- HFE high-fat emulsion -- BCA bicinchoninic acid -- ml/kg bw/d ml/kg body weight/day -- mg/kg bw/d mg/kg body weight/day; Antihyperlipidemic activity -- Antioxidant activity -- Cyclocarya paliurus -- Hepatoprotective effect -- Polysaccharide; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2017.11.033
• Languages: English
• ISSNs: 0144-8617

Abstract

Highlights: CPP-2 was eluted as two main fractions with a weight-average molecular weight of 307 kDa (51.81%) and 3.7 kDa (42.19%). CPP-2 could improve blood lipid levels, liver lipid levels and antioxidant status. CPP-2 showed the antihyperlipidemic, hepatoprotective and antioxidant activities. Abstract: The objective of this study was to analyse the structure of CPP-2, and to observe the pharmacological effects of CPP-2 on lipid metabolism and oxidative stress. CPP-2, eluted as two main fractions comprised of two polysaccharides with Mw of 307 and 3.7 kDa, was mainly consisted of rhamnose, mannose, glucose and galactose in a molar ratio of 1.00:0.78:3.22:0.45. The results showed that treatment with CPP-2 could improve blood lipid levels (TC, TG, HDL-C and LDL-C), liver lipid levels (TC and TG) and antioxidant status (SOD, T-AOC, GSH-PX, MDA and LPO). In addition, the histopathological observations of mice livers and the GPT activities indicated that CPP-2 could attenuate liver cell injury. The present findings demonstrated that CPP-2 might be effective in lowering lipid and protecting against HFE-induced hyperlipidemia and non-alcoholic fatty liver.