1-Hydroxy-3-[(E)-4-(piperazine-diium)but-2-enyloxy]-9, 10-anthraquinone ditrifluoroactate induced autophagic cell death in human PC3 cells. (1st February 2018)

• Record Type: Journal Article
• Authors: Huang, A-Mei; Lin, Kai-Wei; Lin, Wei-Hong; Wu, Li-Hung; Chang, Hao-Chun; Ni, Chujun; Wang, Danny Ling; Hsu, Hsue-Yin; Su, Chun-Li; Shih, Chiaho
• Is Part Of: Chemico-biological interactions. Volume 281(2018)
• Page Start: 60
• Page End: 68
• Publication Date: 2018-02-01
• Subjects: Anthraquinone derivatives -- Autophagy -- ROS -- Prostate cancer; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2017.12.010
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: The autophagy of human prostate cancer cells (PC3 cells) induced by a new anthraquinone derivative, 1-Hydroxy-3-[( E )-4-(piperazine-diium)but-2-enyloxy]-9, 10-anthraquinone ditrifluoroactate (PA) was investigated, and the relationship between autophagy and reactive oxygen species (ROS) generation was studied. The results indicated that PA induced PC3 cell death in a time- and dose-dependent manner, could inhibit PC3 cell growth by G1 phase cell cycle arrest and corresponding decrease in the G2/M cell population and induced S-phase arrest accompanied by a significant decrease G2/M and G1 phase numbers after PC3 cells treated with PA for 48 h, and increased the accumulation of autophagolysosomes and microtubule-associated protein LC3-ll, a marker of autophagy. However, these phenomenon were not observed in the group pretreated with the autophagy inhibitor 3-MA or Bafilomycin A1 (BAF), suggesting that PA induced PC3 cell autophagy. In addition, we found that PA triggered ROS generation in cells, while the levels of ROS decreased in the N-acetylcysteine (NAC) co-treatment, indicating that PA-mediated autophagy was partly blocked by NAC. In summary, the autophagic cell death of human PC3 cells mediated by PA-triggered ROS generation. Highlights: PA a new synthesized anthraquinone showed potent cell growth inhibition in PC3 cells. PA induces autophagy via by increase in LC3-ll levels and addition of BAF in PC3 cells. PA induces autophagy in PC3 cells via ROS and then mediated autophagic cell death.