Glucose monitoring in living cells with single fluorescent protein-based sensors. Issue 5 (10th January 2018)

• Record Type: Journal Article
• Authors: Hu, Hanyang; Wei, Yufeng; Wang, Daocheng; Su, Ni; Chen, Xianjun; Zhao, Yuzheng; Liu, Guixia; Yang, Yi
• Is Part Of: RSC advances. Volume 8:Issue 5(2018)
• Page Start: 2485
• Page End: 2489
• Publication Date: 2018-01-10
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7ra11347a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Glucose is the main source of energy and carbon in organisms and plays a central role in metabolism and cellular homeostasis. Abstract : Glucose is the main source of energy and carbon in organisms and plays a central role in metabolism and cellular homeostasis. However, the sensitive fluctuation of glucose in living cells is difficult to monitor. Thus, we developed a series of ratiometric, highly responsive, single fluorescent protein-based glucose sensors of wide dynamic range by combining a circularly permuted yellow fluorescent protein with a bacterial periplasmic glucose/galactose-binding protein. We used these sensors to monitor glucose transport in living Escherichia coli cells, and found that the cells take up glucose within 10 min to maintain physiological glucose levels, and observed the differences in glucose uptake and glucose metabolism between wild-type and Mlc knockout cells. These sensors can be specific and simple tools for glucose detection in vitro and non-invasive tools for real-time monitoring of glucose metabolism in vivo .