A facile molecularly imprinted polymer-based fluorometric assay for detection of histamine. Issue 5 (9th January 2018)

• Record Type: Journal Article
• Authors: Feng, Xiaotong; Ashley, Jon; Zhou, Tongchang; Halder, Arnab; Sun, Yi
• Is Part Of: RSC advances. Volume 8:Issue 5(2018)
• Page Start: 2365
• Page End: 2372
• Publication Date: 2018-01-09
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7ra11507e
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A new molecularly imprinted polymer (MIP)-based fluorometric assay was developed to directly quantify histamine in real samples. Abstract : Histamine is a biogenic amine naturally present in many body cells. It is also a contaminant that is mostly found in spoiled food. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning. Analytical methods that can routinely screen histamine are thus urgently needed. In this paper, we developed a facile and cost-effective molecularly imprinted polymer (MIP)-based fluorometric assay to directly quantify histamine. Histamine-specific MIP nanoparticles (nanoMIPs) were synthesized using a modified solid-phase synthesis method. They were then immobilized in the wells of a microplate to bind the histamine in aqueous samples. After binding, o -phthaldialdehyde (OPA) was used to label the bound histamine, which converted the binding events into fluorescent signals. The obtained calibration curve of histamine showed a linear correlation ranging from 1.80 to 44.98 μM with the limit of detection of 1.80 μM. This method was successfully used to detect histamine in spiked diary milk with a recovery rate of more than 85%.