Anti-inflammatory effect of chlorogenic acid on the IL-8 production in Caco-2 cells and the dextran sulphate sodium-induced colitis symptoms in C57BL/6 mice. (1st February 2015) - Shin, Hee Soon
Highlights: Chlorogenic acid inhibits TNFα- and H2 O2 -induced IL-8 production in Caco-2 cells. Caffeic acid suppresses the IL-8 production, but not quinic acid. Chlorogenic acid attenuates the DSS-induced colitis symptoms in mice. Chlorogenic acid inhibits MIP-2 and IL-1β mRNA expression induced by DSS. Chlorogenic acid can act as an anti-inflammatory agent for prevention of IBD. Abstract: Chlorogenic acid (CHA) is an antioxidant polyphenol prevalent in human diet, with coffee, fruits, and vegetables being its main source. Effects of CHA and CHA metabolites were evaluated on the IL-8 production in human intestinal Caco-2 cells induced by combined stimulation with tumour necrosis factor alpha (TNFα) and H2 O2 . CHA and caffeic acid (CA) inhibited TNFα- and H2 O2 -induced IL-8 production. We also examined the in vivo effects of CHA and CA using dextran sulphate sodium (DSS)-induced colitis in mice. CHA attenuated DSS-induced body weight loss, diarrhea, fecal blood, and shortening of colon and dramatically improved colitis histological scores. Furthermore, increases in the mRNA expression of colonic macrophage inflammatory protein 2 and IL-1β, which were induced by DSS, were significantly suppressed by CHA supplementation. These results suggest that dietary CHA use may aid in the prevention of intestinal inflammatory conditions.