Specific recognition of Cr3+ under physiological conditions by allyl substituted appendage rhodamine and its cell-imaging studies. Issue 47 (20th November 2017)

• Record Type: Journal Article
• Authors: Bhanja, Anup Kumar; Mishra, Snehasis; Naskar, Kaushik; Maity, Suvendu; Das Saha, Krishna; Sinha, Chittaranjan
• Is Part Of: Dalton transactions. Volume 46:Issue 47(2017)
• Page Start: 16516
• Page End: 16524
• Publication Date: 2017-11-20
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c7dt03560h
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : An Allyl ether-Rhodamine probe emits yellow light (557 nm) in presence of Cr 3+ only with detection limit 33 nM from mixture of other cations and Cr(vi ) in water-CH3 CN medium at pH 7. Imaging of Cr 3+ species in living cells under physiological conditions is achieved. Abstract : An allyloxynitrophenylimio-propylhydroxyl appended rhodamine (RD-3 ) specifically recognises Cr 3+ in aqueous buffer medium at pH 7.4. The visible light excitable reagent ( λ ex 505 nm) on selective binding to Cr 3+ exhibited a strong fluorescence turn-on response with powerful emission at 557 nm and the tail of this luminescence band is extended to ∼650 nm. Interference studies confirm that other metal ions (27 ions) do not hamper the detection process of Cr 3+ . The sensing mechanism is studied by absorption, fluorescence, Job's plot, and 1 H NMR titration studies. The detection limit is as low as 33 nM. The practical applicability ofRD-3 is examined for imaging Cr(iii ) ions in HCT116 (human colorectal carcinoma) cells.