A micropatterning approach to study the influence of actin cytoskeletal organization on polystyrene nanoparticle uptake by BeWo cells. Issue 76 (1st August 2016) - Muoth, C.
Abstract : The microcontact printing technique was successfully applied to study nanoparticle uptake in dependence on different actin cytoskeletal organizations. Abstract : To ensure the safe design and effective application of nanomedical therapies, it is of major importance to understand nanoparticle (NP) uptake mechanisms. Actin-dependent endocytosis has been proposed as a major uptake mechanism for numerous NPs in different cells. However, it is not clear which aspect of the structure and dynamics of the actin cytoskeleton is of relevance in this process. Despite the fact that the cytoskeletal organization is very much dependent on the cellular microenvironment, most mechanistic uptake studies have been performed under classic 2D cell culture conditions, which fail to represent the morphological and mechanical constraints present in biological tissues. In this study, human choriocarcinoma BeWo cells were physically constrained on micropatterns of different geometries to study the uptake of 80 nm, 240 nm and carboxylate 300 nm polystyrene particles and the dependence on different actin structures. Interestingly, major differences in the actin cytoskeletal organization of BeWo cells did not affect the intracellular distribution and amount of internalized polystyrene NPs for all investigated particle sizes and modifications. Therefore, we suggest a more central role for actin dynamics in actin-dependent NP endocytosis, rather than the overall actin organization.