Cryptococcus gattii sero-mating type allelic pattern determined by multiplex PCR. (February 2015)

• Record Type: Journal Article
• Authors: Cogliati, M.; D'Amicis, R.; Tortorano, A.M.
• Is Part Of: Clinical microbiology and infection. Volume 21:Number 2(2015:Feb.)
• Page Start: 190.e1
• Page End: 190.e4
• Publication Date: 2015-02
• Subjects: Cryptococcus gattii -- multiplex PCR -- mating type -- serotype -- molecular type; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2014.09.003
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Molecular methods to differentiate serotypes, mating types and molecular types of Cryptococcus neoformans and C. gattii are important tools to understand epidemiology and pathogenesis of these pathogens. In this study, a multiplex polymerase chain reaction (PCR) approach was applied to sero-mating typing of C. gattii strains. Four pairs of primers were designed to target 4 allele-specific genes located in the mating-type locus. Twenty-three C. gattii strains, presenting different mating types and serotypes, were tested to validate the method. The method was able to identify all sero-mating allelic patterns including hybrid combinations, and therefore, it represents a simple one-step PCR for sero-mating typing of C. gattii strains.