Stem cell behavior on tailored porous oxide surface coatings. (July 2015)

• Record Type: Journal Article
• Authors: Lavenus, Sandrine; Poxson, David J.; Ogievetsky, Nika; Dordick, Jonathan S.; Siegel, Richard W.
• Is Part Of: Biomaterials. Volume 55:(2015)
• Page Start: 96
• Page End: 109
• Publication Date: 2015-07
• Subjects: Nanotopography -- GLAD -- Mesenchymal stem cells -- Porosity -- Titanium dioxide -- Silicon dioxide; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2015.03.033
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Nanoscale surface topographies are known to have a profound influence on cell behavior, including cell guidance, migration, morphology, proliferation, and differentiation. In this study, we have observed the behavior of human mesenchymal stem cells cultured on a range of tailored porous SiO2 and TiO2 nanostructured surface coatings fabricated via glancing angle electron-beam deposition. By controlling the physical vapor deposition angle during fabrication, we could control systematically the deposited coating porosity, along with associated topographic features. Immunocytochemistry and image analysis quantitatively revealed the number of adherent cells, as well as their basic cellular morphology, on these surfaces. Signaling pathway studies showed that even with subtle changes in nanoscale surface structures, the behavior of mesenchymal stem cells was strongly influenced by the precise surface structures of these porous coatings.