Scanning the mitochondrial genome for mutations by cycling temperature capillary electrophoresis. (2nd January 2018)

• Record Type: Journal Article
• Authors: Arstad, Christian; Refinetti, Paulo; Warren, David; Giercksky, Karl-Erik; Ekstrøm, Per Olaf
• Is Part Of: Mitochondrial DNA. Volume 29:Number 1(2018)
• Page Start: 19
• Page End: 30
• Publication Date: 2018-01-02
• Subjects: DNA -- cycling temperature capillary electrophoresis -- mutations -- mitochondrial genome -- cancer
• DOI: 10.1080/24701394.2016.1233532
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: To bypass possible nuclear contamination and to exclusively amplify DNA from the mitochondrion, a set of 23 primers was selected. On the mitochondrial DNA selection fragments, a second set of fragments was used to amplify and identify mutant fractions with a detection limit of 1% . This mutation scanning method analyzed 76% of the mitochondrial genome and was used to examine 94 tumours from different tissues of origin. In all, 87 tumours had one or more mutations, leaving seven samples without observed mutations. Sanger sequencing verified samples carrying mutations with a mutant fraction exceeding 30%. The generated data validate that several regions of the mitochondrial DNA have more mutations than others.