In vitro human adipose-derived stromal/stem cells osteogenesis in akermanite:poly-ɛ-caprolactone scaffolds. (March 2014)

• Record Type: Journal Article
• Authors: Zanetti, Andrea S; McCandless, Gregory T; Chan, Julia Y; Gimble, Jeffrey M; Hayes, Daniel J
• Is Part Of: Journal of biomaterials applications. Volume 28:Number 7(2014:Mar.)
• Page Start: 998
• Page End: 1007
• Publication Date: 2014-03
• Subjects: Akermanite -- poly-ɛ-caprolactone -- scaffolds -- bone -- human adipose-derived stromal/stem cells -- osteogenesis; Biomedical engineering -- Periodicals; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1177/0885328213490974
• Languages: English
• ISSNs: 0885-3282

Abstract

This study compared the metabolic activity, cell proliferation and osteogenic differentiation of human adipose-derived stromal/stem cells cultured on four different scaffolds (poly-ɛ-caprolactone, akermanite:poly-ɛ-caprolactone composites, akermanite and β-tricalcium phosophate) with or without osteogenic media supplementation for up to 21 days. The hypothesis was that human adipose-derived stromal/stem cells osteogenesis in akermanite-containing scaffolds would be greater than the other scaffold types independent of the media supplementation. According to the results, human adipose-derived stromal/stem cells loaded on different scaffolds and cultured in both media conditions displayed significant changes in the metabolic activity and cell proliferation. After 21 days of culture in osteogenic medium, the human adipose-derived stromal/stem cells loaded onto akermanite-based scaffolds had greater calcium deposition and osteocalcin expression relative to human adipose-derived stromal/stem cells loaded onto β-tricalcium phosophate and poly-ɛ-caprolactone. In vivo investigations are needed to further assess the bone tissue engineering potential of human adipose-derived stromal/stem cells loaded to akermanite:poly-ɛ-caprolactone composites.