Protective effects of red grape (Vitis vinifera) juice through restoration of antioxidant defense, endocrine swing and Hsf1, Hsp72 levels in heat stress induced testicular dysregulation of Wister rat. (January 2018)

• Record Type: Journal Article
• Authors: Halder, Soma; Sarkar, Mrinmoy; Dey, Sananda; Kumar Bhunia, Sujay; Ranjan Koley, Alok; Giri, Biplab
• Is Part Of: Journal of thermal biology. Volume 71(2018)
• Page Start: 32
• Page End: 40
• Publication Date: 2018-01
• Subjects: RGJ red grape juice -- CORT Corticosterone -- T Testosterone -- SOD superoxide dismutase -- GSH glutathione -- CAT catalase -- LPO lipid peroxioxidation -- Hsf1 heat shock factor1 gene -- Hsp72 heat shock protein72 gene; Red grape juice -- Vitis vinifera -- Heat stress -- Hyperthermia -- Testis; Thermobiology -- Periodicals; Temperature -- Periodicals; Biology -- Periodicals; Thermobiologie -- Périodiques; Thermobiology; Periodicals; 571.46
• DOI: 10.1016/j.jtherbio.2017.10.011
• Languages: English
• ISSNs: 0306-4565

Abstract

Abstract: Ability of red grape juice (RGJ), a known antioxidant, on testis of adult Wister rat to protect from oxidative stress induced damages by heat stress has been investigated in this study. Heat stress was induced maintaining body and testicular temperature at 43 °C for 30 min/day for 15 days using a hyperthermia induction chamber. Four groups of rats (n=6 per group) comprising of Group-I (control) –kept at 32 °C, Group-II –exposed to heat stress alone, Group-III received RGJ (0.8 ml/rat/day) alone and Group-IV –exposed to heat stress and received RGJ at same dose. Analysis of blood and testicular tissue exhibited significant reduction in serum testosterone, testicular superoxide dismutase, testicular catalase and testicular glutathione (all p < 0.001); whereas, significant rise in the level of serum corticosteroid, testicular lipid peroxidase and the apoptotic enzyme caspase-3 of testis (all p < 0.001) were observed along with substantial increase in testicular Hsp72 and Hsf-1, and decrease in 17β-HSD3 were noted in heat stressed rats compared to controls. In Group-IV rats, RGJ administration could restore these parameters to normal levels. The signs of retention were clear in Group-IV rats and found to be significantly different as compared to that of the Group-II rats. In testicular histology of rats exposed to heat stress alone revealed remarkable germ cell degeneration and tubular deformations which were prevented by RGJ treatment (Group-IV). The reduced number of sperm level in Group-II also restored in RGJ treatment (Group-IV). The above results indicate that consumption of RGJ may substantially protect testis from heat stress induce dysfunctions. Graphical abstract: Highlights: Red grape ( Vitis vinifera ) juice, RGJ, has protective effects against heat stress induced testicular dysregulation. RGJ could protect testis by modulating antioxidant defense and restoring hormonal swing. RGJ restored Hsf1 and Hsp72 gene expression levels and protects testicular cell death during heat stress. Hence, red grape juice could be used as nutritional support to protect body from heat stress related ailments.