Micro‐scale RNA Interference using Iron Oxide Nanoparticle‐modified Lentivirus. Issue 1 (25th October 2017)

• Record Type: Journal Article
• Authors: Liao, Zi‐Xian; Kempson, Ivan M.; Chen, Man‐Jing; Fa, Yu‐Chen; Huang, Kuo‐Yen; Wang, Li‐Fang
• Is Part Of: ChemNanoMat. Volume 4:Issue 1(2018)
• Page Start: 98
• Page End: 102
• Publication Date: 2017-10-25
• Subjects: iron oxide nanoparticles -- lentivirus -- micro-transduction -- RNA interference -- virotherapy; Nanochemistry -- Periodicals; Nanostructured materials -- Periodicals; Nanochemistry; Nanostructured materials; Periodicals; 541.2
• DOI: 10.1002/cnma.201700251
• Languages: English
• ISSNs: 2199-692X

Abstract

Abstract: The US Food and Drug Administration (FDA) has approved the use of virotherapy. However, significant improvements are anticipated if effective localized infection can be achieved. Herein, we demonstrate magnetic lentivirus is guided by magnetic fields. Introduction of short hairpin RNA (shRNA) into the lentivirus genome enabled micro‐scale RNA interference (RNAi) therapy. Although epidermal growth factor receptor (EGFR)‐targeted therapies have successfully treated individuals with non‐small cell lung cancer (NSCLC) cells, clinical therapeutic outcomes have been compromised by acquired resistance to first‐generation tyrosine kinase inhibitors. We assess magnetic lentivirus with NSCLC cells using an in vitro model and achieve micro‐scale RNAi through EGFR silencing. This proof‐of‐principle study demonstrates guided and highly localized micro‐scale, RNAi virotherapy. Abstract : A magnetic lentivirus has been used to achieve micro‐scale RNA interference (RNAi) through EGFR silencing for localized micro‐scale RNAi virotherapy in a NSCLC cell in vitro model.