Ethanol up-regulates phenol sulfotransferase (SULT1A1) and hydroxysteroid sulfotransferase (SULT2A1) in rat liver and intestine. (27th February 2015)

• Record Type: Journal Article
• Authors: Maiti, Smarajit; Chen, Guangping
• Is Part Of: Archives of physiology and biochemistry. Volume 121:Number 2(2015)
• Page Start: 68
• Page End: 74
• Publication Date: 2015-02-27
• Subjects: Ethanol regulation -- Hep G2 cells -- protein expression -- rat liver and intestine -- sulfotransferases; Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.3109/13813455.2014.992440
• Languages: English
• ISSNs: 1381-3455

Abstract

Abstract: Ethanol-consumption impairs physiological-efficiency/endurance, expedites senescence. Impaired-regulations of steroids/biomolecules link these processes. Steroids are catabolized by cytosolic-sulfotransferases (SULTs). Ethanol-induction of eukaryotic-SULTs-expression is scanty. Plant (Brassica-napus) steroid-sulfotransferase; BNST3/BNST4 (gene/BNST) is highly ethanol-inducible (protein/mRNA). Resembling mammalian-SULTs catalytic-mechanism BNSTs show broad substrate-specificities (mammalian-steroids; estradiol/dehydroepiandrosterone/pregnanolone). Recently, ethanol-regulation of SULTs-expression is verified in rat liver/intestine/cultured human-hepatocarcinoma (Hep-G2) cells at enzyme-activity/protein-expression (Western-blot) level. Here, two week's ethanol ingestion by male rat significantly increased SULT2A1 in their liver/intestine ( p < 0.05– p < 0.001) and phenol-sulfotransferase (SULT1A1) in intestine ( p < 0.001) at enzyme-activity/protein levels. In human cells, ethanol significantly (2-fold) increased hSULT1A1/hSULT1E (2–3 fold) protein expressions paralleling their enzymatic-activities ( p < 0.05– p < 0.01). The earlier finding of alcohol-association to the physiological impairment may be corroborated by our present findings. Inductions of SULT-expressions by ethanol have significant physiological/pharmacological consequences.