A COI1 gene in wheat contributes to the early defence response against wheat powdery mildew. Issue 2 (20th November 2017)

• Record Type: Journal Article
• Authors: Liu, Xiaoying; Wang, Junya; Fan, Baoli; Shang, Yuntao; Sun, Yifan; Dang, Chen; Xie, Chaojie; Wang, Zhenying; Peng, Yongkang
• Is Part Of: Journal of phytopathology. Volume 166:Issue 2(2018)
• Page Start: 116
• Page End: 122
• Publication Date: 2017-11-20
• Subjects: early defence response gene -- powdery mildew -- TaCOI1 -- wheat; Plant diseases -- Periodicals; 632
• DOI: 10.1111/jph.12667
• Languages: English
• ISSNs: 0931-1785

Abstract

Abstract: The hormone jasmonate (JA) signalling pathway is involved in diverse developmental processes in plants. COI1 is a key regulator in JA pathway. Although COI1 homologous proteins have been identified in a variety of crops, their regulatory mechanisms in wheat defence responses remain unknown. Here, we cloned COI1 from disease resistant wheat Brock, designated TaCOI1 . TaCOI1 cDNA was 1988‐bp long, with an ORF encoding 593 deduced amino acids. The phylogenetic tree showed that TaCOI1 was clustered in the same clade of monocotyledons. The predicted protein possesses an F‐box domain, several LRRs and a conserved AMN1 domain. Its transcription was induced in the compatible and incompatible wheat by Blumeria graminis f. sp. tritici ( Bgt ) fungus, with differences observed in response time. Expression pattern analysis in the susceptible wheat variety Jing411 revealed that TaCOI1 was up‐regulated after Bgt inoculation at 12‐hour postinoculation (hpi). While, in Brock its transcription was rapidly induced and reached a first peak at 2 hpi. These results suggest that TaCOI1 may be involved in the early response to powdery mildew. The expression of TaCOI1 was decreased by virus‐induced gene silencing. The rate of successful penetration by Bgt was higher in all TaCOI1 ‐silenced Brock, while the percentage of macrocephalic appressoria and resistance reactions was lower than control. These results suggest that silencing of the TaCOI1 gene increased Brock susceptibility to infection. Together, these findings indicate that the TaCOI1 gene could be involved in the early defence response against wheat powdery mildew and may play an important role in wheat– Bgt interactions.