Mycobacterium avium paratuberculosis infection augments innate immune responses following intestinal epithelial injury. (April 2014)

• Record Type: Journal Article
• Authors: Johnson, Charles; Wannemuehler, Michael; Hostetter, Jesse
• Is Part Of: Experimental biology and medicine. Volume 239:Number 4(2014:Apr.)
• Page Start: 436
• Page End: 441
• Publication Date: 2014-04
• Subjects: Dextran sulfate sodium -- innate cytokines -- Mycobacterium avium paratuberculosis -- subclinical infection -- enhanced intestinal inflammation; Physiology -- Periodicals; Biology, Experimental -- Periodicals; Medicine, Experimental -- Periodicals; 610.72
• DOI: 10.1177/1535370213518280
• Languages: English
• ISSNs: 1535-3702

Abstract

We wanted to determine if augmented innate immune activation is associated with lesion development in a mycobacterial enhanced intestinal injury model. We evaluated the local immune response in a Mycobacterium avium paratuberculosis + dextran sulfate sodium ( Map + DSS) model using BALB/c and severe combined immunodeficient (SCID) mice. Map + DSS BALB/c and SCID mice displayed a similar disease phenotype. Moreover, Map + DSS SCID mice had increased expression of interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), inducible nitric oxide synthase (iNOS) and increased numbers of F4/80 positive cells. Additionally, Map antigen is co-localized with iNOS and IL-1β positive cells. This suggests that subclinical Map infection promotes innate immune activation following injury to the intestinal epithelium.